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KMID : 0352419930120030303
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Keimyung Medical Journal
1993 Volume.12 No. 3 p.303 ~ p.309
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Effect of parenteral Administration of Endotoxin on changes of Serum and Hepatic Alkaline Phosphatase and 5'-Nucleotidase Activities in Rats
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Abstract
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The activities of the cytosolic, microsomal and mitochondrial alkaline phosphatase(ALP) and 5'-nucleotidase(5'-NT) in the liver were measured in order to evaluate the cholestatic changes, one of the hepatic damages under the endotoxin
administration.
And the activities of these enzymes in the serum were also measured.
For administration of endotoxin, a dose of 5mg of endotoxin(lipopolysaccharide E. coli 026: B6, from Sigma chemical company, USA) per kg of body weight was administered through a right external jugular vein. Then the rats were killed after 3, 8
and
24
hours of injection with endotoxin to measure the activities of the above enzymes in serum and their livers.
Cytosolic and microsomal ALP and 5'-NT activities showed a significant increase between 3 and 8 hours after endotoxin administration.
The activity of the mitochondrial ALP and 5'-NT in the liver showed no significant changes throughout the experiment.
Serum ALP activity showed a increase without significace. But serum 5'-NT showed a significant increase at 8 hours after endotoxin administration.
According to the results, it is suggested that cytosolic and microsomal ALP and 5'-NT activity is increased caused by increased biosynthesis and impairment of hepatic excretory function for these enzymes. And it is also suggested that increased
activity
of the serum ALP and 5'-NT is due to the reflux of these enzymes form the liver and due to leak into the blood through the damaged membrane of hepatocyte.
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